Biology · IGCSE 0610 · §12.1–12.3

Respiration

How cells release the energy in nutrient molecules, with or without oxygen.

Biology · 0610 Topic 12 of 18

Uses of Energy

RespirationReleasing energy from nutrient moleculesUses of energyWhy cells need it;yeast and temperatureAerobicUses oxygenAnaerobicNo oxygen;much less energyOxygen debtEXT onlytogetherCells break down nutrient molecules, such as glucose,to release the energy that every process needswith oxygen (aerobic) or without it (anaerobic)
FIG 12.0 How the chapter connects: Every living cell respires. The reactions release energy from nutrient molecules, using oxygen in aerobic respiration and no oxygen in anaerobic respiration.

Living organisms need energy to stay alive. The energy comes from respiration, which takes place in every living cell.

Definition
Respiration
The chemical reactions in cells that break down nutrient molecules to release energy.
GOnutrients and oxygen release energyrespiration in yeastrespiration in muscles
FIG 12.7 Respiration releases energy in cells, including yeast and muscle.
Use of energyWhat happens
Muscle contractionMuscles shorten, causing movement
Protein synthesisCells build proteins
Cell divisionCells divide to make new cells
Active transportSubstances are moved across membranes
GrowthOrganisms increase in size
Passage of nerve impulsesMessages travel along nerves
Maintenance of a constant body temperatureHeat is released to keep the body warm

Worked example (naming the use): A student runs for a bus. Name the use of energy in the leg muscles. Step 1. The leg muscles shorten to move the legs. Step 2. This use of energy is muscle contraction. Answer: Muscle contraction.

Respiration goes on all the time

Respiration takes place in every living cell, all the time, not only during exercise. Cells that are very active, such as muscle cells, release energy at a faster rate than cells that are resting. The energy is used for the processes in the table above.

Worked example (naming a use): A mammal keeps its body at the same temperature on a cold day. Name the use of energy involved. Step 1. The body releases heat from respiration to stay warm. Step 2. This use of energy is the maintenance of a constant body temperature. Answer: Maintaining a constant body temperature.

Examiner note
Energy is released in respiration, not made. Respiration is not the same as breathing.
Why this matters
Every process in a living organism needs energy, so respiration must go on all the time.

Respiration in Yeast

Yeast is a single-celled organism. In a solution of glucose it respires and releases carbon dioxide, which can be seen as bubbles.

water bath at a set temperatureyeast +glucosebubbles of carbon dioxidedelivery tubeyeast and glucose solutioncount bubbles per minute
FIG 12.1 Apparatus to investigate respiration in yeast.

Method

Put equal volumes of yeast and glucose solution into tubes, and place each tube in a water bath at a different temperature, for example from 20 to 60 °C. Leave the tubes for a few minutes to reach the temperature. Count the bubbles of gas released in the same time, for example one minute, and repeat to find a mean. Keep everything except the temperature the same.

highest raterate risesrate fallstemperaturerate of respiration
FIG 12.2 Rate of respiration in yeast at different temperatures.

Result

As the temperature rises, the rate of respiration increases up to a highest rate. At higher temperatures the rate falls.

Examiner note
Name the independent variable (temperature), the dependent variable (bubbles per minute) and what you keep the same: volume of mixture, mass of yeast and glucose concentration.
Examiner note
Quote the pattern: rate increases with temperature up to a highest rate, then falls.
Why this matters
Yeast is a convenient organism for investigating respiration, because the carbon dioxide it releases is easy to see.

Aerobic Respiration

In aerobic respiration, nutrient molecules such as glucose are broken down using oxygen, releasing energy.

Definition
Aerobic respiration
The chemical reactions in cells that use oxygen to break down nutrient molecules to release energy.
glucose+oxygenreleases energycarbon dioxide+waternutrient moleculefrom the air, via blood
FIG 12.3 The word equation for aerobic respiration.
SubstanceWhere it comes from or goes
GlucoseFrom digested food, carried in the blood
OxygenFrom the air, carried in the blood
Carbon dioxideReleased and removed by the lungs
WaterA product of the reactions

ExtendedBalanced chemical equation

C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O

One molecule of glucose reacts with six molecules of oxygen to give six molecules of carbon dioxide and six molecules of water.

Worked example (checking the balance): Show that the balanced equation has the same number of oxygen atoms on each side. Step 1. Left side: 6 in glucose plus 6 × 2 = 12 in oxygen gives 18. Step 2. Right side: 6 × 2 = 12 in carbon dioxide plus 6 in water gives 18. Answer: 18 = 18.

Examiner note
Learn the word equation exactly. Glucose and oxygen are the reactants; carbon dioxide and water are the products.
Why this matters
Aerobic respiration releases much more energy per glucose than the anaerobic kind.

Anaerobic Respiration

In anaerobic respiration, nutrient molecules are broken down without oxygen. It releases much less energy per glucose molecule than aerobic respiration.

Definition
Anaerobic respiration
The chemical reactions in cells that break down nutrient molecules to release energy without using oxygen.
in yeastglucosealcohol+carbon dioxidein muscles during vigorous exerciseglucoselactic acid
FIG 12.4 Word equations for anaerobic respiration.
OrganismWord equation
Yeastglucose → alcohol + carbon dioxide
Muscles (vigorous exercise)glucose → lactic acid

ExtendedBalanced equation in yeast

C₆H₁₂O₆ → 2C₂H₅OH + 2CO₂

One glucose molecule gives two molecules of alcohol (ethanol) and two molecules of carbon dioxide.

Worked example (choosing the equation): Which products form when muscles respire anaerobically during vigorous exercise? Step 1. The word equation for muscles is glucose gives lactic acid. Step 2. No carbon dioxide is formed in this equation. Answer: Lactic acid.

Examiner note
Say anaerobic respiration releases much less energy, not no energy. The products depend on the organism.
Why this matters
Muscles can keep working for a short time when oxygen cannot be supplied fast enough.

Comparing Respiration

Both kinds of respiration break down nutrient molecules such as glucose to release energy, but they differ in several ways.

FeatureAerobicAnaerobic
OxygenUsedNot used
Energy released per glucoseMuch moreMuch less
Products in yeastCarbon dioxide and waterAlcohol and carbon dioxide
Products in musclesCarbon dioxide and waterLactic acid
aerobicanaerobicmuch moremuch lessenergy per glucose
FIG 12.5 Energy released from one glucose molecule (not to scale).

Anaerobic respiration releases much less energy per glucose molecule than aerobic respiration. Aerobic respiration is the main way that cells release energy.

Worked example (comparing the two): Give one similarity and one difference between aerobic respiration and anaerobic respiration in muscles. Step 1. Similarity: both break down glucose to release energy. Step 2. Difference: aerobic uses oxygen and releases much more energy; anaerobic does not use oxygen. Answer: Same fuel; oxygen and energy differ.

Examiner note
In a comparison, give both sides: aerobic uses oxygen and releases much more energy; anaerobic does not use oxygen and releases much less.
Why this matters
Cells use aerobic respiration whenever oxygen is available, because it releases far more energy.

The Oxygen Debt

ExtendedHow the debt arises

During vigorous exercise the muscles also respire anaerobically. Lactic acid builds up in the muscles and blood, causing an oxygen debt.

Definition
Oxygen debt
(Extended) The extra oxygen needed after vigorous exercise to break down the lactic acid that has built up.
oxygen neededoxygen taken inrestvigorous exerciserecoveryoxygen debtrepaidtimeoxygen taken in (rate)
FIG 12.6 Oxygen taken in during and after vigorous exercise (idealised).

ExtendedHow the debt is removed

(a) The heart rate stays fast, to transport lactic acid in the blood from the muscles to the liver.

(b) Breathing stays deeper and faster, to supply oxygen for aerobic respiration of the lactic acid.

(c) The lactic acid is broken down by aerobic respiration in the liver.

Worked example (explaining recovery): Explain why a runner keeps breathing heavily after a race has ended. Step 1. Lactic acid built up in the muscles during anaerobic respiration. Step 2. Oxygen is needed to break the lactic acid down by aerobic respiration in the liver. Step 3. Deeper, faster breathing supplies this oxygen. Answer: Repaying the oxygen debt.

Examiner note
Give all three parts: fast heart rate carries lactic acid to the liver; deeper, faster breathing supplies oxygen; the liver respires the lactic acid aerobically.

Exam advice

Common mistakes

Confusing respiration with breathing
Breathing moves air in and out of the lungs. Respiration is the release of energy in cells.
Saying energy is “made” or “produced”
Respiration releases energy that is stored in nutrient molecules.
Saying anaerobic respiration releases no energy
It releases much less energy per glucose than aerobic respiration, not none.
Writing the wrong numbers in a balanced equation
C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O. Check that each side has the same number of atoms.
Saying lactic acid is removed in the muscles
It is carried to the liver and broken down there by aerobic respiration.

Model answer

Describe an investigation of the effect of temperature on respiration in yeast.
[4 marks]
Mark 1
[app] The set-up.
Put equal volumes of yeast and glucose solution in tubes in water baths at different temperatures (for example 20 to 60 °C).
Mark 2
[app] The measurement.
Count the bubbles of carbon dioxide released in a set time.
Mark 3
[app] Control.
Keep the volume of mixture, mass of yeast and glucose concentration the same.
Mark 4
[k] The result.
The rate increases with temperature up to a highest rate, then falls.

Recall checklist

  • Define respiration and list the uses of energy.
  • Describe the yeast investigation and its result.
  • Define aerobic respiration; give its word equation.
  • Define anaerobic respiration; give both word equations.
  • Compare the energy released by the two kinds.
  • Write the balanced equations (Extended)
  • State that lactic acid builds up and causes an oxygen debt (Extended)
  • Outline how the oxygen debt is removed (Extended)

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